Today, We learned about the ELISA tests and Secondary Antibody tests. We performed a test to determine the purity of a meat sample by poking little holes into agar and injecting bovine and hamburger solutions with Anti-Bovine horse, goat, and pig solutions, to see which ones would react. We also did a control with red and green dye. Then we performed the ELISA test used to detect hepatitis and HIV. First, you coat the bottom with an antigen, rinse with a buffer wash, then add a positive antibody solution, negative antibody solution, and other samples. After washing, add a secondary antibody solution, let sit, rinse, then add a substrate that will change color to indicate a positive test. It was really cool to see tests that are used to detect such serious viruses. We also smelled essential oils that we will be utilizing in our research projects. We also saw that our bacteria that was stored in the freezer had grown when inoculated on an agar plate! 
Hello Dude,
ReplyDeleteThanks for sharing your views about Secondary Antibody. These antibodies are useful to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation...
pSIVA