Last week, our group split the tasks of acid fast and endospore staining our unknown samples. I completed the acid fast stain by using Ziehl-Neelson carbolfuchsin on a slide that was over a beaker of boiling water. After decolorizing that stain with acid-alcohol and covering it with methylene blue for two minutes, I used the microscope's oil immersion lens to determine that our unknown sample is non-acid fast, because the sample was purple. Annie also determined our unknown sample (L 25) had no endospores. 
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