Today, we made a tiny sample of our bacteria to store long term in the -80 degree freezer until the end of the semester. Using a pipettor, we combined 700 microliters of our environmental bacterial broth sample from last week and 300 microliters of the cryoprotectant glycerol. Then to determine the oxygen requirements of our unknown bacteria, we performed two different tests. First, we inoculated our sample in a thioglycollate broth, which turns pink when oxygen is present. We will determine the oxygen requirements next Thursday after examination. Then we made a streak plate of the unknown sample to use in the GasPak Anaerobic system. The GasPak eliminates oxygen using a chemical reaction and produces carbon dioxide. An indicator strip inside the GasPak turns blue when oxygen is present, and stays colorless when oxygen is absent. We will check those results Thursday as well. Enjoy the pictures!
Today, we made a tiny sample of our bacteria to store long term in the -80 degree freezer until the end of the semester. Using a pipettor, we combined 700 microliters of our environmental bacterial broth sample from last week and 300 microliters of the cryoprotectant glycerol. Then to determine the oxygen requirements of our unknown bacteria, we performed two different tests. First, we inoculated our sample in a thioglycollate broth, which turns pink when oxygen is present. We will determine the oxygen requirements next Thursday after examination. Then we made a streak plate of the unknown sample to use in the GasPak Anaerobic system. The GasPak eliminates oxygen using a chemical reaction and produces carbon dioxide. An indicator strip inside the GasPak turns blue when oxygen is present, and stays colorless when oxygen is absent. We will check those results Thursday as well. Enjoy the pictures!
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